Waters new xevo g2xs qtof mass spectrometer, bringing. Qtof 2 utilises a high performance, research grade quadrupole mass analyser, incorporating a prefilter assembly to protect the main analyser from contaminating deposits, and an orthogonal acceleration time of flight tof mass spectrometer. Xevo g2s tof instrument specifications 2 vacuum system differentially pumped, automated vacuum system comprising aircooled turbomolecular pumps and one backing pump either one rotary pump or one oil free pump. Xevo g2 qtof is equipped with a dual electrospray probe ion source that can spray a sample lc inlet and a calibration standard solution lock spray in a rapidly alternating manner. We have 1 waters xevo g2 xs qtof manual available for free pdf download. Acquity uplc h class system coupled to a xevo g2 tof mass spectrometer waters corp. Considerations specific to the xevo g2 s tof solventleakage hazard the source exhaust system is designed to be robust and leaktight. Our expert automation engineers will find, configure and offer the best devices for your application.
Metabolites of glibenclamide detected using %a %b 0 98 e2 5. Intact quantification of trastuzumab is demonstrated using the xevo g2xs qtof hrms and unifi scientific information system. Sep 14, 2011 watch this xevo g2 qtof animation to learn how you can obtain the highest quality, most comprehensive information that will enable you to make the right decisions quickly and confidently. June 2011 720003448en ao pdf ms sensitivity esi the peak at mz 503 from a solution of 500 pgl raffinose in 7030 acetonitrilewater no additives, will have an intensity of greater than 1728 counts per second. Intended use of the xevo g2 xs qtof waters designed the orthogonal acceleration, timeofflight xevo g2 xs qtof for use as a research tool to deliver authenticated mass measurement. We have 1 waters xevo g2xs qtof manual available for free pdf download. Follow the procedure in your instruments overview and maintenance guidefor example, in section 7. Our expert automation engineers will find, configure and offer the best devices for. Xevo g2 tof delivers exceptional levels of sensitivity, selectivity, inspectrum dynamic range, speed of analysis, quantitative accuracy and exact mass. The tof g2 s sample kit 1 is intended for the verification of system performance for tof g2 s. View and download waters xevo g2 qtof operators, overview and maintenance manual online. In this report, the ems cell retrofitted into a hybrid qtof tandem. Wat introduces a new data acquisition mode, sonar, for its xevo g2xs quadrupole timeofflight qtof mass spectrometer.
Waters advances proteomics and lipidomics research with novel. The xevo g2xs qtof delivers not only conventional ms and msms methods of data acquisition, but also mse for comprehensive accurate mass precursor and fragment ion information from a single analysis, fastdda for rapid, intelligent automated accurate mass msms, and tofmrm for enhanced quantitative capability. Uplcmse and quantof technology, offers unparalleled sensitivity with. Uplcqtofms analysis of extracts from the leaves of. Jun 16, 2014 the xevo g2 xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tof mrm, stepwave tm and quantof tm to deliver optimum levels of robust. The analysis of the dichloromethane subfractions was performed on a waters xevo g2 q tof mass spectrometer waters ms technologies, manchester, uk, which was equipped with an electrospray ionization interface with waters acquity uhplc system waters, co. Comparison of drug detection by three quadrupole timeof. A waters acquity uplc system waters, milford, ma, usa was interfaced to a hybrid quadrupoleorthogonal acceleration tof mass spectrometer xevo g2 qtof, waters micromass, manchester, uk, using an orthogonal zsprayesi interface operating in both positive and negative ionisation modes. Ionbench with the waters xevo g2xs qtof mass spectrometer. Waters acquity hclass uplc with pda and column manager. Please contact us for complete waters refurbishedused mass spectrometer system, warranty and service contract details. In this report, the ems cell retrofitted into a hybrid q tof tandem. Intended use of the xevo g2xs qtof waters designed the orthogonal acceleration, timeofflight xevo g2xs qtof for use as a research tool to deliver authenticated mass measurement. The mz transmission range for a quadrupole in nonresolving mode is 20 to 32,000 mz for the 8000 mz quadrupole.
Uplcqtofms analysis of extracts from the leaves of pouteria. Xevo g2s tof is designed for scientists who need to identify and quantify the broadest range of compounds in complex and challenging samples. Use of different highresolution mass spectrometry platforms. The waters xevo g2 q tof is coupled to a waters acquity uplc iclass, that offers the most powerful solutions to complex separation challenges. Concerning the convenience of the xevo g2 s qtof, i can say that the use of masslynx and targetlynx software provided by waters for targeted analysis was relatively practical and can afford a. The xevo g2xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tofmrm, stepwavetm and quantoftm to deliver optimum levels of robust sensitivity and selectivity, resulting in the detection of more lowlevel compounds than ever before.
Xevo source door is hard to open wkb52906 last updated. Electroncapturedissociationofsodiumadductedpeptides on a. Your quantitative and qualitative questions answered. Waters xevo g2 qtof xevo g2 qtof is the most sensitive, exactmass, quantitative and qualitative benchtop msms system available. Refurbished waters xevo g2 qtof pn 186005597 complete with esi source, pump and masslynx pc workstation. Q tof 2 utilises a high performance, research grade quadrupole mass analyser, incorporating a prefilter assembly to protect the main analyser from contaminating deposits, and an orthogonal acceleration time of flight tof mass spectrometer. Intact trastuzumab monoclonal antibody quantification using the. The xevo g2xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tofmrm, stepwave tm. Overview to scale the picture below is a simulation at scale of the thermo tsq quantis ms system right side connected with a vanquish uhplc left side, installed on a ionbench ms mass spec bench or table the vacuum pump sogevac sv65bi is enclosed in the noise reduction cabinet, under your mass spectrometer thermo tsq quantis. Ms analysis was performed on a xevo g2 qtof waters ms technologies, manchester, uk. Analysis was performed using waters xevo g2 s q tof and thermo qexactive mass spectrometers equipped with suitable uplc systems. The tof mass range is 20 to 100,000 mz in resolution mode, and 20 to 32,000 mz in high resolution mode. Online iexms characterization and monitoring of mab charge.
It delivers the integrat ed workflow benefits of engineered simplicity while incorporating the groundbreaking quantof technology of the synapt g2 system. Watch this xevo g2 qtof animation to learn how you can obtain the highest quality, most comprehensive information that will enable you to make the right decisions quickly and confidently. Vacuum read backs and system ventpump cycles are digitally monitored and controlled, to provide total software. Request pdf quantitation by high resolution mass spectrometry.
The xevo g2xs qtof combines stepwave ion optics, xs. Stepwave ion optics provides unsurpassed levels of durable sensitivity. Waters xevo g2s bench top qtof, designed for identification and confirmation of the broadest range of compounds in complex and challenging samples, is used for studying proteins and their complexes. Antibody quantification using the xevo g2xs qtof high resolution mass. In the ewre lab we have a watersmicromass xevo g2 xs qtof, quadrupole timeofflight mass spectrometer. Waters advances proteomics and lipidomics research with. Concerning the convenience of the xevo g2s qtof, i can say that the use of masslynx and targetlynx software provided by waters for targeted analysis was relatively practical and can afford a. Intact trastuzumab monoclonal antibody quantification. Qtof 2 users guide micromass uk limited floats road. The analysis of the dichloromethane subfractions was performed on a waters xevo g2 qtof mass spectrometer waters ms technologies, manchester, uk, which was equipped with an electrospray ionization interface with waters acquity uhplc system waters, co. The xevo g2 xs qtof is for research use only and is not intended for use in diagnostic applications. A hexapole collision cell, between the two mass analysers, can be.
Uplcqtofmsbased phenolic profiling of melastomaceae. Determination of the chemical components and phospholipids of. Xevo g2 s tof instrument specifications 2 vacuum system differentially pumped, automated vacuum system comprising aircooled turbomolecular pumps and one backing pump either one rotary pump or one oil free pump. Waters xevo g2xs qtof overview and maintenance manual pdf. All three platforms performed well with 90% of compounds detected in one set of spiked plasma samples, and 7988% for a second set.
The tof g2s sample kit 1 is intended for the verification of system performance for tof g2s. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2 xs tof provides maximum robustness with no compromise in performance, the highest quality, most comprehensive qualitative and quantitative information. Tof mrm, on the synapt g2s hdms and xevo g2xs mass spectrometers. The xevo g2 xs tof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. The instrument is developed to give the most accurate and reproducible separation of complex sample matrices. In 2008, the present authors described an electromagnetostatic ems cell that can be retrofitted into virtually any existing type of mass spectrometer 16. Comparison of different time of flightmass spectrometry. Quantof technology delivers superior uplccompatible mass resolution, matrixtolerant dynamic range, quantitative. The xevo g2 xs qtof delivers not only conventional ms and msms methods of data acquisition, but also mse for comprehensive accurate mass precursor and fragment ion information from a single analysis, fastdda for rapid, intelligent automated accurate mass msms, and tof mrm for enhanced quantitative capability. Uplcqtofmsbased phenolic profiling of melastomaceae, their. Xevo g2 xs tof performance, robustness, and flexibility made accessible to everyone. Overview to scale the picture below is a simulation at scale of the waters xevo g2xs qtof lcms mass spectrometer system right side connected with an acquity uhplc left side, installed on a ionbench ms mass spec bench or table. Data were acquired in profile mode with masslynx software, version 4. Squirt methanol into the hinge joint and manually work the hinge back and forth to free movement.
Q3, qtof or orbitrap lcms which will be better for. Xevo g2 xs tof is a benchtop time of flight ms combining stepwave ion optics, xs collision cell and quantof technology for superior robustness, sensitivity and selectivity, resulting in high quality, comprehensive qualitative and quantitative information. Waters xevo g2xs qtof manuals manuals and user guides for waters xevo g2xs qtof. Nov 15, 2019 follow the procedure in your instruments overview and maintenance guidefor example, in section 7. Jun 16, 2014 the xevo g2 xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tof mrm, stepwavetm and quantoftm to deliver optimum levels of robust sensitivity and selectivity, resulting in the detection of more lowlevel compounds than ever before. Intact quantification of trastuzumab is demonstrated using the xevo g2 xs qtof hrms and unifi scientific information system. A waters acquity uplc system waters, milford, ma, usa was interfaced to a hybrid quadrupoleorthogonal accelerationtof mass spectrometer xevo g2 qtof, waters micromass, manchester, uk, using an orthogonal zsprayesi interface operating in both positive and negative ionisation modes.
How to rebuild the reference probe of a xevo or synapt tof ms. Intact trastuzumab monoclonal antibody quantification using. The xevo g2xs qtof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comp rehensive qualitative and quantitative information. Featuring the new xs collision cell, the xevo g2xs qtof stands alone among its peers in offering. The proteus ecometer s is an ultrasonic liquid level sensor, battery powered and equipped with a digital radio transmitter. This kit is designed for use by a waters certified engineer or user as part of a performance maintenance or qualification activity. Xevo g2xs tof performance, robustness, and flexibility made accessible to everyone.
Ionbench with the thermo tsq quantis mass spectrometer. The waters xevo g2 qtof is coupled to a waters acquity uplc iclass, that offers the most powerful solutions to complex separation challenges. A hexapole collision cell, between the two mass analysers, can be used to induce. Comprehensive characterization of lignans from forsythia. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% lc eluate. Ion mobility and liquid chromatographymass spectrometry. Vacuum read backs and system ventpump cycles are digitally monitored and controlled, to provide total software control and ensure failsafe operation in the event of power failure. See the whole picture with the new 7250 gcqtof duration. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2xs tof provides maximum robustness with no compromise in performance, the highest quality, most comprehensive qualitative and quantitative information. An acquity uplc iclass system coupled to a waters xevo g2xs qtof mass spectrometer detector waters uk. How to rebuild the reference probe of a xevo or synapt tof.
For scientists who need to confidently identify and quantify unknown compounds in complex samples, the xevo g2 tof mass spectrometer with uplcmse and quantof technology offers exceptional levels of selectivity, inspectrum dynamic range, speed. Engineered for standard biotherapeutic characterization analyses, the x500b qtof system is a true benchtop instrument, with streamlined processing to get to a consistent, accurate view of your biologic. This is designed for use with both gas and liquid sample inlet device, specifically a dedicated uplc and api gc. Waters xevo g2 qtof xevo g2 qtof is the most sensitive, exactmass, quantitative and. The bench or supporting surface must be vibration free. Xevo g2 qtof laboratory equipment pdf manual download. Uhplcqtof ms screening of pharmaceuticals and their. A xevo g2s qtof waters, milford, ma, usa was used to obtain. What is the latest version of masslynx software for the. The instrument incorporates the newest technologies in the area of ion optics for increased sensitivity. Overview to scale the picture below is a simulation at scale of the waters xevo g2 xs qtof lcms mass spectrometer system right side connected with an acquity uhplc left side, installed on a ionbench ms mass spec bench or table. Analysis was performed on a waters xevo g2xs qtof equipped with acquity uplci class and unifi software. The xevo g2xs qtof is for research use only and is not intended for use in diagnostic applications. The following table may help you calculate the additional btus of heat dissipation from this new equipment.
Formation of 3 mcpd fatty acid esters from monostearoyl. What is the latest version of masslynx software for the xevo g2 xs qtof and xevo g2 xs tof. Part 1 of 3 transforming high performance lcms analysis in. During long analyses, critical hardware components stay clean for longer, so results are more reproducible and the laboratory stays productive. The xevo g2xs tof combines stepwave ion optics with the xs collision cell, providing a significant increase in sensitivity with no reduction in selectivity. Waters introduces xevo g2xs qtof and xevo tqs micro mass. Xevo g2xs tof the xevo g2xs tof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. Maximums represent the heat given off when heated zones are set for maximum temperatures.
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